The Transplantation Immunology Division (TID) at Hoxworth Blood Center is a state of the art histocompatibility and immunogenetics testing laboratory, that supports busy clinical transplant programs and transplantation research activities.
TID provides testing and consultation support services for both solid-organ (kidney, liver, pancreas, heart) and hematopoietic stem cell transplant programs located throughout the Greater Cincinnati area. This includes the solid organ transplant programs at The University Hospital , The Christ Hospital, and Cincinnati Children’s Hospital Medical Center (CCHMC), as well as two hematopoietic stem cell transplant programs at CCHMC and The Jewish Hospital. Both CCHMC and The Jewish Hospital combined are responsible for more than 100 allogeneic stem cell transplants yearly. This level of overall activity places the Hoxworth TID laboratory in the top 25% of all HLA laboratories in terms of testing volumes*.
TID delivers state-of-the-art testing in histocompatibility and immunogenetics to assist area physicians and hospitals with immune monitoring, clinical trials, and disease diagnosis to a large group of patients. The Hoxworth TID is accredited by the American Society of Histocompatibility and Immunogenetics (ASHI), HHS under the Clinical Laboratory Improvement Act (CLIA), and works within the context of the blood center’s compliance with current good manufacturing practices (cGMP) concurrent with working in an FDA-regulated environment.
Immunogenetics and histocompatibility testing
The TID uses DNA-based molecular diagnostic techniques to identify HLA genes at low/intermediate or high-level allele resolution. HLA-A, -B, -C, -DR, -DQ and -DP locus types at an intermediate level of resolution are provided by reverse SSO (Sequence-Specific Oligonucleotides) hybridization.
Furthermore, PCR-SSP (Polymerase Chain Reaction-Sequence-Specific Primers) is used either for rapid (3-4 hours) determination of HLA-A, -B, -C, -DR and DQ typing at low resolution or to provide higher resolution testing.
Sequence-based typing (microcapillary sequencer) allows the highest resolution typing for HLA-A, -B, -C, -DR, -DQ (alpha and beta) and -DP alleles. In addition to HLA alleles, TID provides typing for other human histocompatibility antigens (MICA = MHC class I chain-related gene A; KIR = Killer Cell Ig-Like Receptors).
Test turnaround times are: