Immunohematology Reference Laboratory
Immunohematology Reference Laboratory (IRL) Services
Hoxworth Blood Center’s Immunohematology Reference Laboratory provides testing and consultation to assist hospitals in solving patients’ red blood cell (RBC) serological problems. The IRL provides rare (antigen matched) blood by maintaining local liquid and frozen rare blood inventories and by participating in the American Rare Donor Program (ARDP), a national search service. The IRL is an AABB and CLIA accredited Immunohematology Reference Laboratory (IRL). CLIA # 36D0346846.
Laboratory Hours: 8:00 am 12:00 am, Monday Thursday, 8:00 am - 4:30 pm, Friday. On-Call staff are available 24/7 during non-staffed hours (weekdays, holidays and weekends) to provide laboratory testing services and confirmed typed RBC units for STAT requests and to provide historical antigen negative (non-confirm typed) RBC units.
Lab Contact Numbers:
Laboratory Phone: 513-558-1547
On-Call Phone: 513-558-1539 (request IRL Tech paged)
Fax Number: 513-558-1533
Technical Supervisor:
Kathleen Larimore, MS, MT(ASCP)SBB
Asst. Director, IRL
Ph: 513-558-1549
Division Director, Laboratories:
Gregg Boothe, MS, MT(ASCP)SBB
Ph: 513-558-1200. Press "1". Say "2". Say "Gregg Boothe". You will be automatically connected.
Medical Director:
Patricia Carey, MD
Ph: 513-558-1200. Press "1". Say "2". Say "Patricia Carey". You will be automatically connected.
Service Offered:
Below is a list of available tests and services that can be requested. All IRL testing requires completion of test requisition. Click here for a IRL Consultation Request Form (J1001-1).
Red Cell Antibody Investigations
- Single to Complex Multiple Antibodies
- Auto-antibodies
- Drug-dependant Antibodies
- Antibodies to High- and Low-Incidence Antigens
- Reagent-dependant Reactivity
Hemolytic Disease of the Newborn Investigations
Transfusion Reaction Investigations
Aberrant or Discrepant Result Investigations
Poly agglutination Investigations
Donor/ Patient Red Cell Phenotype Requests
Prenatal evaluations including titers
Procurement of Antigen-Negative Donor Units
Routine testing performed by IRL uses in-date FDA licensed reagents and kits when available. Non-licensed reagents may be required in complex evaluations when licensed reagents are not available. Suitable controls are tested when using non-licensed reagents.
Sample Requirements
Optimal sample size for red cell serological workups: 30 mL EDTA anticoagulated whole blood. See consultation request form for specific requirements.
Turnaround Times (TAT)
Depending on the complexity of the work up and the request status, turn around times will vary. The following guidelines are used by the IRL:
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REQUEST LEVEL |
TURN AROUND TIME FOR VERBAL REPORT |
|
STAT (Life Threatening) |
8 hours from time of sample receipt |
|
ASAP (Transfusion Needed but not Life Threatening) |
24 hours from time of sample receipt |
|
Routine |
1 - 5 days from sample receipt |
Turnaround time listed above is from the time the sample is received in the testing laboratory. The timeframes listed above are guidelines. TAT may vary by the complexity and extent of the serological problem.
GUIDELINES FOR EMERGENCY STATUS
The following questions are designed to help identify referrals for immunohematologic resolution that should be elevated to an emergency (STAT) workup.
- Patient’s clinical symptoms:
a. High output failure: heart failure-rapid beating with insufficient O2 delivery.
b. Angina: coronary artery disease with persistent decrease in O2 delivery to myocardial muscle.
c. Impending stroke: cerebral vascular disease with persistent decrease in O2 delivery to cerebrum.
- Is the patient actively bleeding? Yes / No
Note: a rapid drop in hemoglobin (> 1 gm/dL / 24 hours) places the patient at risk.
- Does the patient require surgery during the next 24 hours? Yes / No
- Are all units incompatible? Yes / No
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Emergency status should be considered for those patients who: |
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Suffer any of the symptoms listed in #1. Or
Have a yes answer to question 2. Or
Have a yes answer to questions 3 and 4. |
Turn around time for reporting results (verbally or by fax) on a STAT request is, generally, within 8 hours of sample receipt. Please remember to complete, as thoroughly as possible, the IRL Consultation Request Form (J1001-1), and contact Hoxworth Blood Center IRL personnel before sending samples.
REPORTING AND BILLING
Preliminary reports (prior to final review) and billing information will be phoned and faxed* at completion of workup.
Final reports will be mailed and faxed* to the hospital after final review has been completed.
Billing invoice will be forwarded to your facility by Hoxworth Blood Center Financial Services. The submitting facility is responsible for all charges and/or fees related to the resolution of the patient’s serologic problem and provision of RBC units.
*Faxing of reports and bills is done if requested by the facility. Reports and bills contain Protected Health Information (PHI) and can only be faxed to the confirmed number(s) provided by the facility’s authorized person(s).
Updated: 10/30/09
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