Recent Publications
Clinical Research in Transfusion Medicine
Molecular mechanisms controlling HSC engraftment and mobilization
Extrinsic and small GTPase signaling in cancer stem cells and progenitors
Research Publications
Patents
Grant Support
Clinical Research in Transfusion Medicine
Selected Publications
1. Evaluation of proposed FDA criteria for the evaluation of radiolabeled red cell recovery trials. Dumont LJ, AuBuchon JP, for the Biomedical Excellence for Safer Transfusion (BEST) Collaborative. (Cancelas J, Rugg N, Hoxworth Blood Center, participating laboratory). Transfusion 2008 Jun 48(6):1053-60.
2. Successful in vivo recovery and extended storage of additive solution (AS)-5 red blood cells after deglycerolization and resuspension in AS-3 for 15 days with an automated closed system. Bandarenko N, Cancelas JA, Snyder E, Haya SN, Rugg N, Corda T, Joines AD, Gormas JF, Pratt PG, Kowalskya R, Rose M, Rose L, Foley J, Popovsky M. Transfusion 2007 Apr;47:680-6.
Molecular mechanisms controlling HSC engraftment and mobilization
Selected Publications
1. Cdc42 critically regulates the balance between myelopoiesis and erythropoiesis. Yang L, Wang L, Kalfa TA, Cancelas JA, Shang X, Pushkaran S, Mo J, Williams DA, Zheng Y. Blood 2007 Dec 1;110(12):3853-61.
2. Rac GTPases differentially integrate signals regulating hematopoietic stem cell localization. Cancelas JA, Lee AW, Prabhakar R, Stringer KF, Zheng Y, Williams DA. Nat Med 2005 Aug;11(8):886-91.
Extrinsic and small GTPase signaling in cancer stem cells and progenitors.
Selected Publications
1. FIP1L1/PDGFRalpha synergizes with SCF to induce systemic mastocytosis in a murine model of chronic eosinophilic leukemia/hypereosinophilic syndrome. Yamada Y, Sanchez-Aguilera A, Brandt EB, McBride M, Al-Moamen NJ, Finkelman FD, Williams DA, Cancelas JA, Rothenberg ME. Blood 2008 Sep 15;112(6):2500-7.
2. Rac guanosine triphosphatases represent integrating molecular therapeutic targets for BCR-ABL-induced myeloproliferative disease. Thomas EK, Cancelas JA, Chae HD, Cox AD, Keller PJ, Perrotti D, Neviani P, Druker BJ, Setchell KD, Zheng Y, Harris CE, Williams DA. Cancer Cell 2007 Nov;12(5):467-78.
Research Publications
1. Nf1 mutation expands an EGFR-dependent peripheral nerve progenitor that confers neurofibroma tumorigenic potential. Williams JP, Wu J, Johansson G, Rizvi TA, Miller SC, Geiger H, Malik P, Li W, Mukouyama YS, Cancelas JA, Ratner N. Cell Stem Cell. 2008 Dec 4;3(6):658-69. PMID: 19041782
2. Rac guanosine triphosphatases represent a potential target in AML. Müller LU, Schore RJ, Zheng Y, Thomas EK, Kim MO, Cancelas JA, Gu Y, Williams DA. Leukemia. 2008 Sep;22(9):1803-6. Epub 2008 Jul 31. PMID: 18668135
3. Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development. Guo F, Cancelas JA, Hildeman D, Williams DA, Zheng Y. Blood. 2008 Sep 1;112(5):1767-75. Epub 2008 Jun 25. PMID: 18579797
4. FIP1L1/PDGFRalpha synergizes with SCF to induce systemic mastocytosis in a murine model of chronic eosinophilic leukemia/hypereosinophilic syndrome. Yamada Y, Sanchez-Aguilera A, Brandt EB, McBride M, Al-Moamen NJ, Finkelman FD, Williams DA, Cancelas JA, Rothenberg ME. Blood. 2008 Sep 15;112(6):2500-7. Epub 2008 Jun 6. PMID: 18539901
5. Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia. Wei J, Wunderlich M, Fox C, Alvarez S, Cigudosa JC, Wilhelm JS, Zheng Y, Cancelas JA, Gu Y, Jansen M, Dimartino JF, Mulloy JC. Cancer Cell. 2008 Jun;13(6):483-95. PMID: 18538732
6. Rho GTPases and regulation of hematopoietic stem cell localization. Williams DA, Zheng Y, Cancelas JA. Methods Enzymol. 2008;439:365-93. Review. PMID: 18374178
7. Tissue inhibitor of metalloproteinase-3 via oncolytic herpesvirus inhibits tumor growth and vascular progenitors. Mahller YY, Vaikunth SS, Ripberger MC, Baird WH, Saeki Y, Cancelas JA, Crombleholme TM, Cripe TP. Cancer Res. 2008 Feb 15;68(4):1170-9. PMID: 18281493
8. Plexiform and dermal neurofibromas and pigmentation are caused by Nf1 loss in desert hedgehog-expressing cells. Wu J, Williams JP, Rizvi TA, Kordich JJ, Witte D, Meijer D, Stemmer-Rachamimov AO, Cancelas JA, Ratner N. Cancer Cell. 2008 Feb;13(2):105-16. PMID: 18242511
9. Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells. Ghiaur G, Ferkowicz MJ, Milsom MD, Bailey J, Witte D, Cancelas JA, Yoder MC, Williams DA. Blood. 2008 Apr 1;111(7):3313-21. Epub 2007 Dec 14. PMID: 18083846
10. Rac guanosine triphosphatases represent integrating molecular therapeutic targets for BCR-ABL-induced myeloproliferative disease. Thomas EK, Cancelas JA, Chae HD, Cox AD, Keller PJ, Perrotti D, Neviani P, Druker BJ, Setchell KD, Zheng Y, Harris CE, Williams DA. Cancer Cell. 2007 Nov;12(5):467-78. PMID: 17996650
11. Cdc42 critically regulates the balance between myelopoiesis and erythropoiesis. Yang L, Wang L, Kalfa TA, Cancelas JA, Shang X, Pushkaran S, Mo J, Williams DA, Zheng Y. Blood. 2007 Dec 1;110(12):3853-61. Epub 2007 Aug 16. PMID: 17702896
12. Genetic and pharmacologic evidence that Rac1 GTPase is involved in regulation of platelet secretion and aggregation. Akbar H, Kim J, Funk K, Cancelas JA, Shang X, Chen L, Johnson JF, Williams DA, Zheng Y. J Thromb Haemost. 2007 Aug;5(8):1747-55. PMID: 17663742
13. Successful in vivo recovery and extended storage of additive solution (AS)-5 red blood cells after deglycerolization and resuspension in AS-3 for 15 days with an automated closed system. Bandarenko N, Cancelas J, Snyder EL, Hay SN, Rugg N, Corda T, Joines AD, Gormas JF, Pratt GP, Kowalsky R, Rose M, Rose L, Foley J, Popovsky MA. Transfusion. 2007 Apr;47(4):680-6. PMID: 17381627
14. Rho GTPase Cdc42 coordinates hematopoietic stem cell quiescence and niche interaction in the bone marrow. Yang L, Wang L, Geiger H, Cancelas JA, Mo J, Zheng Y. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5091-6. Epub 2007 Mar 12. PMID: 17360364
15. Leukaemia: niche retreats for stem cells. Williams DA, Cancelas JA. Nature. 2006 Dec 14;444(7121):827-8. PMID: 17167463
16. Stem cell collection and gene transfer in Fanconi anemia. Kelly PF, Radtke S, von Kalle C, Balcik B, Bohn K, Mueller R, Schuesler T, Haren M, Reeves L, Cancelas JA, Leemhuis T, Harris R, Auerbach AD, Smith FO, Davies SM, Williams DA. Mol Ther. 2007 Jan;15(1):211-9. PMID: 17164793
17. Buffering and dilution in red blood cell storage. Hess JR, Rugg N, Joines AD, Gormas JF, Pratt PG, Silberstein EB, Greenwalt TJ. Transfusion. 2006 Jan;46(1):50-4. PMID: 16398730
18. Children are not little adults: just ask their hematopoietic stem cells. Williams DA, Xu H, Cancelas JA. J Clin Invest. 2006 Oct;116(10):2593-6. PMID: 17016556
19. Viability of red cells prepared with S-303 pathogen inactivation treatment. Rios JA, Hambleton J, Viele M, Rugg N, Sindermann G, Greenwalt T, Wages D, Cook D, Corash L. Transfusion. 2006 Oct;46(10):1778-86. PMID: 17002635
20. In vitro and in vivo evaluation of LEUKOSEP HRC-600-C leukoreduction filtration system for red cells. AuBuchon JP, Cancelas JA, Herschel L, Roger J, Rugg N, Pratt PG, Joines A, Gormas JF, Zia M, Spearman MR. Transfusion. 2006 Aug;46(8):1311-5. PMID: 16934065
21. The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization. Cancelas JA, Jansen M, Williams DA. Exp Hematol. 2006 Aug;34(8):976-85. Review. PMID: 16863904
22. Structure-function based design of small molecule inhibitors targeting Rho family GTPases. Nassar N, Cancelas J, Zheng J, Williams DA, Zheng Y. Curr Top Med Chem. 2006;6(11):1109-16. Review. PMID: 16842149
23. Inhibition of RhoA GTPase activity enhances hematopoietic stem and progenitor cell proliferation and engraftment. Ghiaur G, Lee A, Bailey J, Cancelas JA, Zheng Y, Williams DA. Blood. 2006 Sep 15;108(6):2087-94. Epub 2006 May 18. PMID: 16709932
24. Hints to blood groupers, 1950. Schmidt PJ, Greenwalt TJ. Transfusion. 2006 Mar;46(3):448-53. PMID: 16533289
25. Bone marrow connexin-43 expression is critical for hematopoietic regeneration after chemotherapy. Presley CA, Lee AW, Kastl B, Igbinosa I, Yamada Y, Fishman GI, Gutstein DE, Cancelas JA. Cell Commun Adhes. 2005 Jul-Dec;12(5-6):307-17. PMID: 16531325
26. Stem cell mobilization by beta2-agonists. Cancelas JA, Williams DA. Nat Med. 2006 Mar;12(3):278-9. No abstract available. PMID: 16520770
27. Rational design and applications of a Rac GTPase-specific small molecule inhibitor. Akbar H, Cancelas J, Williams DA, Zheng J, Zheng Y. Methods Enzymol. 2006;406:554-65. PMID: 16472687
28. The FIP1L1-PDGFRA fusion gene cooperates with IL-5 to induce murine hypereosinophilic syndrome (HES)/chronic eosinophilic leukemia (CEL)-like disease. Yamada Y, Rothenberg ME, Lee AW, Akei HS, Brandt EB, Williams DA, Cancelas JA. Blood. 2006 May 15;107(10):4071-9. Epub 2006 Jan 17. PMID: 16418325
29. The how and why of exocytic vesicles. Greenwalt TJ. Transfusion. 2006 Jan;46(1):143-52. Review. PMID: 16398744
30. Antibodies, antigens, and anticoagulants: a historical review of a lifetime in transfusion medicine--the Landsteiner Lecture 2004. Greenwalt TJ. Transfusion. 2005 Sep;45(9):1531-9. PMID: 16131388
31. Interlaboratory comparison of red-cell ATP, 2,3-diphosphoglycerate and haemolysis measurements. Hess JR, Kagen LR, van der Meer PF, Simon T, Cardigan R, Greenwalt TJ, AuBuchon JP, Brand A, Lockwood W, Zanella A, Adamson J, Snyder E, Taylor HL, Moroff G, Hogman C. Vox Sang. 2005 Jul;89(1):44-8. PMID: 15938739
32. A rapid method for retrovirus-mediated identification of complementation groups in Fanconi anemia patients. Chandra S, Levran O, Jurickova I, Maas C, Kapur R, Schindler D, Henry R, Milton K, Batish SD, Cancelas JA, Hanenberg H, Auerbach AD, Williams DA. Mol Ther. 2005 Nov;12(5):976-84. Epub 2005 Aug 9. PMID: 16084127
33. Rac GTPases differentially integrate signals regulating hematopoietic stem cell localization. Cancelas JA, Lee AW, Prabhakar R, Stringer KF, Zheng Y, Williams DA. Nat Med. 2005 Aug;11(8):886-91. Epub 2005 Jul 17. PMID: 16025125
34. Rac2-deficient hematopoietic stem cells show defective interaction with the hematopoietic microenvironment and long-term engraftment failure. Jansen M, Yang FC, Cancelas JA, Bailey JR, Williams DA. Stem Cells. 2005 Mar;23(3):335-46. PMID: 15749928
35. My reminiscence of the early days of the American Red Cross Rare Donor Registry. Greenwalt TJ. Immunohematology. 2004;20(4):253-4. PMID: 15679459
36. Collection of two units of leukoreduced RBCs from a single donation with a portable multiple-component collection system. Snyder EL, Elfath MD, Taylor H, Rugg N, Greenwalt TJ, Baril L, Whitley P, Brantigan B, Story K. Transfusion. 2003 Dec;43(12):1695-705. PMID: 14641866
37. Hematopoietic cell regulation by Rac1 and Rac2 guanosine triphosphatases. Gu Y, Filippi MD, Cancelas JA, Siefring JE, Williams EP, Jasti AC, Harris CE, Lee AW, Prabhakar R, Atkinson SJ, Kwiatkowski DJ, Williams DA. Science. 2003 Oct 17;302(5644):445-9. PMID: 14564009
38. Twelve-week RBC storage. Hess JR, Hill HR, Oliver CK, Lippert LE, Rugg N, Joines AD, Gormas JF, Pratt PG, Silverstein EB, Greenwalt TJ. Transfusion. 2003 Jul;43(7):867-72. PMID: 12823745
39. Storage of red blood cells: new approaches. Hess JR, Greenwalt TG. Transfus Med Rev. 2002 Oct;16(4):283-95. Review. PMID: 12415514
40. Biochemical and structural changes in RBCs stored with different plasticizers: the role of hexanol. Draper CJ, Greenwalt TJ, Dumaswala UJ. Transfusion. 2002 Jul;42(7):830-5. PMID: 12375654
41. Alkaline CPD and the preservation of RBC 2,3-DPG. Hess JR, Hill HR, Oliver CK, Lippert LE, Greenwalt TJ. Transfusion. 2002 Jun;42(6):747-52. PMID: 12147028
42. Evaluation of fructose diphosphate in RBC preservation. Greenwalt TJ, Gormas JF, Rugg N, Rios J, Goldsmith JC, Prokopczuk E, Hardin CD, Roberts TM. Transfusion. 2002 Mar;42(3):384-5.
43. Myeloablation enhances engraftment of transduced murine hematopoietic cells, but does not influence long-term expression of the transgene. Puig T, Kádár E, Limón A, Cancelas JA, Eixarch H, Luquín L, García M, Barquinero J. Gene Ther. 2002 Nov;9(21):1472-9. PMID: 12378410
44. Procoagulant activity of T lymphoblastoid cells due to exposure of negatively charged phospholipid. Barrowcliffe TW, Fábregas P, Jardi M, Cancelas J, Rabaneda M, Felez J. Thromb Haemost. 2002 Mar;87(3):442-9. PMID: 11916077
45. RBC storage for 11 weeks. Hess JR, Rugg N, Gormas JK, Knapp AD, Hill HR, Oliver CK, Lippert LE, Silberstein EB, Greenwalt TJ. Transfusion. 2001 Dec;41(12):1586-90. PMID: 11778076
46. The effects of polyvinyl chloride and polyolefin blood bags on red blood cells stored in a new additive solution. Hill HR, Oliver CK, Lippert LE, Greenwalt TJ, Hess JR. Vox Sang. 2001 Oct;81(3):161-6. PMID: 11703858
47. The role of electrolytes and pH in RBC ASs. Hess JR, Rugg N, Knapp AD, Gormas JF, Hill HR, Oliver CK, Lippert LE, Greenwalt TJ. Transfusion. 2001 Aug;41(8):1045-51. PMID: 11493737
48. To test or not to test for syphilis: a global problem. Greenwalt TJ, Rios JA. Transfusion. 2001 Aug;41(8):976. PMID: 11493726
49. The effect of two additive solutions on the postthaw storage of RBCs. Hess JR, Hill HR, Oliver CK, Lippert LE, Greenwalt TJ. Transfusion. 2001 Jul;41(7):923-7. PMID: 11452161
50. Successful storage of RBCs for 10 weeks in a new additive solution. Hess JR, Rugg N, Knapp AD, Gormas JF, Silberstein EB, Greenwalt TJ. Transfusion. 2000 Aug;40(8):1012-6. PMID: 10960531
51. Successful storage of RBCs for 9 weeks in a new additive solution. Hess JR, Rugg N, Knapp AD, Gormas JF, Silberstein EB, Greenwalt TJ. Transfusion. 2000 Aug;40(8):1007-11. PMID: 10960530
52. The effects of phosphate, pH, and AS volume on RBCs stored in saline-adenine-glucose-mannitol solutions. Hess JR, Lippert LE, Derse-Anthony CP, Hill HR, Oliver CK, Rugg N, Knapp AD, Gormas JF, Greenwalt TJ. Transfusion. 2000 Aug;40(8):1000-6. PMID: 10960529
Patents
Prolonged Storage of Red Blood Cells and Composition Hess JR, Greenwalt TJ
Novel additive solutions that are useful for the storage of human red blood cells (RBCs) under refrigerated conditions. Also, a method of using the additive solutions in an appropriate volume to preserve RBCs at about 1 to 6°C for up to 10 weeks. The solutions and processes allow the viable storage of human RBCs for an extended period of time in a solution which is directly infusible in humans.
Issue Date: November 21, 2000 US Patent #6,150,085
Preservation and Shelf Life Extension of Blood Cells Hess JR, Greenwalt TJ
Preservative solutions that extend the useful shelf-life of red cells from the current 6 to 12 weeks. This intellectual property is shared by the US Army and the University of Cincinnati. Implementation is expected to save 600,000 units from outdating annually, to reduce seasonal shortages of blood, and to make autologous donor programs more efficient.
Issue Date: 11/20/01 US Patent #6,447,987
Grant Support
1. “Rac GTPases Inhibition in Chronic Myelogenous Leukemia” Pending NIH R01 NS238850 (PI Cancelas)
2. “Inhibition of Rac GTPases in Chronic Myelogenous Leukemia Therapy” Jan 2007-Dec 2010 Dept of Defense (PI Cancelas).
3. “Rac GTPases in BCR-ABL Acute Lymphoblastic Leukemia” Alex’s Lemonade Stand Grant Jul 2007-Jun 2009. (PI Cancelas)
4. “Transcriptional Control of Respiratory Epithelial Progenitor Cells”. NIH R01 Oct 2007-Sep 2012. (PI Whitsett, Co-PI Cancelas)
5. “Mitogenic Activities in Neurofibromatosis” EGFR in neurofibromatosis NIH 2006-2011 (PI Ratner, Co-Investigator Cancelas).
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